Tetrapods move through various environments using a diversity of locomotor behaviours, including flying, swimming, walking and running. Associated with these behaviours are suites of musculoskeletal specializations that have evolved numerous times is distantly related taxa, suggesting the mechanical demands associated with movement greatly influence and could even be predicted by musculoskeletal structure. Our lab studies how in vivo locomotor mechanics influence musculoskeletal development in both closely and distantly related taxa performing similar or vastly different locomotor behaviours both in the lab and in natural environments. The goal of this work is to understand how the diversity of musculoskeletal loading patterns among vertebrates influence the development and evolution of different morphologies, including structural and tissue properties and architecture.